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CLEC14A Recombinant antibody

CLEC14A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241206D3

Cat no : 84327-1-RR

Print datasheet

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

验证数据展示

  • WB analysis using 84327-1-RR

    WB analysis using 84327-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84327-1-RR (CLEC14A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84327-1-RR

    Affinity and Kinetic Characterization of 84327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84327-1-RR against Human CLEC14A were performed. The affinity constant is 8.76 nM.

经过测试的应用

Positive WB detected inHEK-293T cells, HUVEC cells, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84327-1-RR is CLEC14A Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CLEC14A fusion protein Ag21081 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name C-type lectin domain family 14, member A
Synonyms UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27
Calculated Molecular Weight 490 aa, 52 kDa
Observed Molecular Weight 52 kDa, 100 kDa
GenBank Accession NumberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

实验方案

Product Specific Protocols
WB protocol for CLEC14A antibody 84327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols