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验证数据展示

  • WB analysis using 84331-4-RR

    WB analysis using 84331-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84331-4-RR

    IHC staining of mouse kidney using 84331-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 84331-4-RR

    IF Staining of Jurkat using 84331-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84331-4-RR

    Affinity and Kinetic Characterization of 84331-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

RAPGEF2 Recombinant antibody

RAPGEF2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84331-4-RR

产品说明书

CloneNo. 241637G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84331-4-RR targets RAPGEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RAPGEF2 fusion protein Ag35288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF) 2
别名 PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF
计算分子量167kDa
观测分子量200 kDa
GenBank蛋白编号NM_014247.2
基因名称 RAPGEF2
Gene ID (NCBI) 9693
RRIDAB_3671872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y4G8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
IHC protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
IF protocol for RAPGEF2 antibody 84331-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols