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TSG101 Recombinant antibody

TSG101 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241657A1

Cat no : 84334-4-RR

Print datasheet

Synonyms

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

验证数据展示

  • WB analysis using 84334-4-RR

    WB analysis using 84334-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84334-4-RR

    IHC staining of human colon cancer using 84334-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSG101 antibody included
  • Affinity and Kinetic Characterization of 84334-4-RR

    Affinity and Kinetic Characterization of 84334-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, JurKat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84334-4-RR is TSG101 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TSG101 fusion protein Ag28569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor susceptibility gene 101
Synonyms VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for TSG101 antibody 84334-4-RRDownload protocol
IHC protocol for TSG101 antibody 84334-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols