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验证数据展示

  • WB analysis using 84387-6-RR

    WB analysis using 84387-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84387-6-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84387-6-RR

    IF Staining of HepG2 using 84387-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD98/SLC3A2 Recombinant antibody

CD98/SLC3A2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84387-6-RR

产品说明书

CloneNo. 241755A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CD98/SLC3A2 Ready-To-Use IHC Kit. CD98/SLC3A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84387-6-RR targets CD98/SLC3A2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
别名 CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain
计算分子量68kDa
观测分子量68-125 kDa
GenBank蛋白编号NM_002394.5
基因名称 CD98
Gene ID (NCBI) 6520
RRIDAB_3671923
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08195-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).

实验方案

Product Specific Protocols
WB protocol for CD98/SLC3A2 antibody 84387-6-RRDownload protocol
IF protocol for CD98/SLC3A2 antibody 84387-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols