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Serum amyloid P component Recombinant antibody, PBS Only (Detector)

Serum amyloid P component Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241403F5

Cat no : 84402-1-PBS

Print datasheet

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

验证数据展示

  • WB analysis of human plasma using 84402-1-RR (same clone as 84402-1-PBS)

    WB analysis of human plasma using 84402-1-RR (same clone as 84402-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 84402-1-RR (Serum amyloid P component antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84402-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01275-1

    Sandwich ELISA standard curve of MP01275-1

    Sandwich ELISA standard curve of MP01275-1, Human APCS Recombinant Matched Antibody Pair - PBS only. 84402-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0528. 84402-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

  • Sample test of MP01275-1

    Sample test of MP01275-1

    Serum of eight individual healthy human donors was measured. The human APCS concentration of detected samples was determined to be 32.91 µg/mL with a range of 16.86 - 48.68 µg/mL.

  • Affinity and Kinetic Characterization of 84402-1-RR

    Affinity and Kinetic Characterization of 84402-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84402-1-RR against Human Serum amyloid P component were performed. The affinity constant is 1.49 nM.

产品信息

84402-1-PBS targets Serum amyloid P component as part of a matched antibody pair:

MP01275-1: 84402-2-PBS capture and 84402-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name amyloid P component, serum
Synonyms APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02743
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.