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验证数据展示

  • WB analysis of mouse placenta using 84412-3-RR

    WB analysis of mouse placenta using 84412-3-RR

    mouse placenta tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of bEnd.3 using 84412-3-RR

    IF Staining of bEnd.3 using 84412-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD141/Thrombomodulin antibody (84412-3-RR, Clone: 241730D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84412-3-RR

    Affinity and Kinetic Characterization of 84412-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84412-3-RR against Mouse CD141/Thrombomodulin were performed. The affinity constant is 3.11 nM.

CD141/Thrombomodulin Recombinant antibody

CD141/Thrombomodulin Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84412-3-RR

产品说明书

CloneNo. 241730D4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

TM, Thrombomodulin, Thbd, Fetomodulin, CD141

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse placenta tissue, mouse lung tissue
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84412-3-RR targets CD141/Thrombomodulin in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thrombomodulin
别名 TM, Thrombomodulin, Thbd, Fetomodulin, CD141
计算分子量62kDa
观测分子量105 kDa
GenBank蛋白编号NM_009378.3
基因名称 Thbd
Gene ID (NCBI) 21824
RRIDAB_3671941
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 84412-3-RRDownload protocol
IF protocol for CD141/Thrombomodulin antibody 84412-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols