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验证数据展示

  • WB analysis using 84413-3-RR

    WB analysis using 84413-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 84413-3-RR

    IHC staining of human colon using 84413-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCL20/MIP3A antibody included
  • IHC staining of human colon using 84413-3-RR

    IHC staining of human colon using 84413-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84413-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCL20/MIP3A antibody included

CCL20/MIP-3 alpha Recombinant antibody

CCL20/MIP-3 alpha Recombinant Antibody for WB, IHC, ELISA
Cat No. 84413-3-RR

产品说明书

CloneNo. 241694B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, RAW 264.7 cells, HepG2 cells, HuH-7 cells, SMMC-7721 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCL20/MIP3A Ready-To-Use IHC Kit. CCL20/MIP3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1250-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84413-3-RR targets CCL20/MIP-3 alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 20
别名 CCL20, MIP-3 Alpha, MIP-3α, CCL 20, C-C motif chemokine 20
计算分子量11kDa
观测分子量11 kDa
GenBank蛋白编号NM_004591.3
基因名称 CCL20/MIP-3 alpha
Gene ID (NCBI) 6364
RRIDAB_3671942
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78556-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

实验方案

Product Specific Protocols
WB protocol for CCL20/MIP-3 alpha antibody 84413-3-RRDownload protocol
IHC protocol for CCL20/MIP-3 alpha antibody 84413-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols