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ZNHIT2 Recombinant antibody

ZNHIT2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241868B10

Cat no : 84418-7-RR

Print datasheet

Synonyms

ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10

验证数据展示

  • WB analysis using 84418-7-RR

    WB analysis using 84418-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84418-7-RR (ZNHIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84418-7-RR

    Affinity and Kinetic Characterization of 84418-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84418-7-RR against Human ZNHIT2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells, HeLa cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84418-7-RR targets ZNHIT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ZNHIT2 fusion protein Ag10351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc finger, HIT type 2
Synonyms ZNHIT 2, Zinc finger HIT domain-containing protein 2, FON, C11orf5, 241868B10
Calculated Molecular Weight 403 aa, 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC052240
Gene Symbol ZNHIT2
Gene ID (NCBI) 741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UHR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNHIT2 belongs to a family of HIT-finger proteins characterized by the presence of a HIT domain, a roughly 50-residue zinc-binding domain containing conserved cysteine (Cys) and histidine (His) residues. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP. ZNHIT2 is highly expressed in testis.

实验方案

Product Specific Protocols
WB protocol for ZNHIT2 antibody 84418-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols