• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-PLCG2 (Tyr1217) Recombinant antibody

Phospho-PLCG2 (Tyr1217) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240631F2

Cat no : 84456-1-RR

Print datasheet

Synonyms

p- PLCγ2, p PLCγ2, p PLCG2 (Tyr1217), p PLCγ2 (Tyr1217), Phospho PLCG2 (Tyr1217)

验证数据展示

  • WB analysis using 84456-1-RR

    WB analysis using 84456-1-RR

    Non-treated and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 84456-1-RR (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inpervanadate treated Ramos cells
Planning an IHC experiment? We recommend our IHCeasy PLCG2 Ready-To-Use IHC Kit. PLCG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84456-1-RR targets Phospho-PLCG2 (Tyr1217) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Synonyms p- PLCγ2, p PLCγ2, p PLCG2 (Tyr1217), p PLCγ2 (Tyr1217), Phospho PLCG2 (Tyr1217)
Calculated Molecular Weight 148 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

实验方案

Product Specific Protocols
WB protocol for Phospho-PLCG2 (Tyr1217) antibody 84456-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols