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P2RX6 Recombinant antibody

P2RX6 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241620B10

Cat no : 84458-4-RR

Print datasheet

Synonyms

P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor

验证数据展示

  • WB analysis of mouse skeletal muscle using 84458-4-RR

    WB analysis of mouse skeletal muscle using 84458-4-RR

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84458-4-RR (P2RX6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84458-4-RR

    Affinity and Kinetic Characterization of 84458-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84458-4-RR against Human P2RX6 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84458-4-RR targets P2RX6 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen P2RX6 fusion protein Ag12331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 6
Synonyms P2XM, P2X6, P2X purinoceptor 6, P2RXL1, ATP receptor
Calculated Molecular Weight 431 aa, 48 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC033488
Gene Symbol P2RX6
Gene ID (NCBI) 9127
RRIDAB_3671983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)

实验方案

Product Specific Protocols
WB protocol for P2RX6 antibody 84458-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols