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CD100 Recombinant antibody

CD100 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241708D10

Cat no : 84465-4-RR

Print datasheet

Synonyms

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

验证数据展示

  • IF Staining of Jurkat using 84465-4-RR

    IF Staining of Jurkat using 84465-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84465-4-RR

    Affinity and Kinetic Characterization of 84465-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

经过测试的应用

Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84465-4-RR targets CD100 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Synonyms SEMA4D, SEM4D, CD100 antigen, CD 100, A8
Calculated Molecular Weight 96 kDa
GenBank Accession NumberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

实验方案

Product Specific Protocols
IF protocol for CD100 antibody 84465-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols