验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, COLO 205 cells, PC-3 cells, HT-1080 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84476-6-RR targets ADAMTS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ADAMTS2 fusion protein Ag34957 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase with thrombospondin type 1 motif, 2 |
| 别名 | ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2 |
| 计算分子量 | 135 kDa |
| 观测分子量 | 110~130 kDa |
| GenBank蛋白编号 | NM_014244 |
| 基因名称 | ADAMTS2 |
| Gene ID (NCBI) | 9509 |
| RRID | AB_3671997 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O95450 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS2 antibody 84476-6-RR | Download protocol |
| IF protocol for ADAMTS2 antibody 84476-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |