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验证数据展示

  • WB analysis using 84491-6-RR

    WB analysis using 84491-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84491-6-RR (PQBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84491-6-RR

    IF Staining of HeLa using 84491-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84491-6-RR

    Affinity and Kinetic Characterization of 84491-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84491-6-RR against Human PQBP1 were performed. The affinity constant is 0.529 nM.

PQBP1 Recombinant antibody

PQBP1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84491-6-RR

产品说明书

CloneNo. 241901B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PQBP-1, Polyglutamine-binding protein 1, Polyglutamine tract-binding protein 1, Npw38, 241901B11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, mouse pancreas tissue, rat brain tissue, rat thymus tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PQBP1 Ready-To-Use IHC Kit. PQBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84491-6-RR targets PQBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PQBP1 fusion protein Ag9329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polyglutamine binding protein 1
别名 PQBP-1, Polyglutamine-binding protein 1, Polyglutamine tract-binding protein 1, Npw38, 241901B11
计算分子量 265 aa, 30 kDa
观测分子量 30-32 kDa
GenBank蛋白编号BC012358
基因名称 PQBP1
Gene ID (NCBI) 10084
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60828
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

实验方案

Product Specific Protocols
WB protocol for PQBP1 antibody 84491-6-RRDownload protocol
IF protocol for PQBP1 antibody 84491-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols