• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LMO7 Recombinant antibody, PBS Only (Capture)

LMO7 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241781D11

Cat no : 84495-4-PBS

Print datasheet

Synonyms

LOMP, FBX20, 241781D11

验证数据展示

  • WB analysis using 84495-4-RR (same clone as 84495-4-PBS)

    WB analysis using 84495-4-RR (same clone as 84495-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84495-4-RR (LMO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84495-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01336-4

    Sandwich ELISA standard curve of MP01336-4

    Sandwich ELISA standard curve of MP01336-4, Human LMO7 Recombinant Matched Antibody Pair - PBS only. 84495-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30016. 84495-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01336-4

    Sample test of MP01336-4

    The mean LMO7 concentration was determined to be 70.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 99.53 ng/mL in mouse lung tissue extract based on a 1.6 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84495-4-RR

    Affinity and Kinetic Characterization of 84495-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84495-4-RR against Human LMO7 were performed. The affinity constant is 0.246 nM.

产品信息

84495-4-PBS targets LMO7 as part of a matched antibody pair:

MP01336-4: 84495-4-PBS capture and 84495-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen LMO7 fusion protein Ag30016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name LIM domain 7
Synonyms LOMP, FBX20, 241781D11
Calculated Molecular Weight 193 kDa
Observed Molecular Weight150 kDa, 180 kDa
GenBank Accession NumberNM_005358
Gene Symbol LMO7
Gene ID (NCBI) 4008
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WWI1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).