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验证数据展示

  • WB analysis of L02 using 84527-4-RR

    WB analysis of L02 using 84527-4-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84527-4-RR

    WB analysis using 84527-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84527-4-RR

    WB analysis using 84527-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84527-4-RR

    Affinity and Kinetic Characterization of 84527-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84527-4-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

FADS2-Specific Recombinant antibody

FADS2-Specific Recombinant Antibody for WB, ELISA
Cat No. 84527-4-RR

产品说明书

CloneNo. 241863D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, Jurkat cells, mouse liver tissue, LNCaP cells, A549 cells, PC-3 cells, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84527-4-RR targets FADS2-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FADS2-Specific fusion protein Ag27715 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fatty acid desaturase 2
别名 FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase
计算分子量 445 aa, 49 kDa
观测分子量45 kDa
GenBank蛋白编号BC009011
基因名称 FADS2
Gene ID (NCBI) 9415
RRIDAB_3672034
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FADS2-Specific antibody 84527-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols