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IL1RN Recombinant antibody, PBS Only (Capture)

IL1RN Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241879G2

Cat no : 84532-5-PBS

Print datasheet

Synonyms

IL 1ra, IL-1RA, IL1RA, IL 1RN, ICIL-1RA

验证数据展示

  • Sandwich ELISA standard curve of MP01389-3

    Sandwich ELISA standard curve of MP01389-3

    Sandwich ELISA standard curve of MP01389-3, Human IL-1RA Recombinant Matched Antibody Pair - PBS only. 84532-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1277. 84532-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IL-1RA concentration was determined to be 4,505.9 pg/mL in unstimulated PBMC supernatant, 74,132.2 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IL-1RA, and measured 534.4 pg/mL.

  • Sample test of MP01389-3

    Sample test of MP01389-3

    Serum of six individual healthy human donors was measured. The IL-1RA concentration of detected samples was determined to be 637.3 pg/mL with a range of 49.7-1,417.8 pg/mL.

产品信息

84532-5-PBS targets IL1RN as part of a matched antibody pair:

MP01389-3: 84532-5-PBS capture and 84532-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IL1RN fusion protein Ag1277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1 receptor antagonist
Synonyms IL 1ra, IL-1RA, IL1RA, IL 1RN, ICIL-1RA
Calculated Molecular Weight 20 kDa
GenBank Accession NumberBC009745
Gene Symbol IL-1RA
Gene ID (NCBI) 3557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18510
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C