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MDMX/MDM4 Recombinant antibody

MDMX/MDM4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241936A3

Cat no : 84534-6-RR

Print datasheet

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

验证数据展示

  • IF Staining of U2OS using 84534-6-RR

    IF Staining of U2OS using 84534-6-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 84534-6-RR

    IF Staining of A549 using 84534-6-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inA549 cells, U2OS cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84534-6-RR targets MDMX/MDM4 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MDMX/MDM4 fusion protein Ag26678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Mdm4 p53 binding protein homolog (mouse)
Synonyms MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX
Calculated Molecular Weight 490 aa, 55 kDa
GenBank Accession NumberBC067299
Gene Symbol MDMX/MDM4
Gene ID (NCBI) 4194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for MDMX/MDM4 antibody 84534-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols