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FFAR2 Recombinant antibody

FFAR2 Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241696B4

Cat no : 84544-1-RR

Print datasheet

Synonyms

GPCR43, G protein coupled receptor 43, free fatty acid receptor 2, FFA2R, FFA2

验证数据展示

  • WB analysis using 84544-1-RR

    WB analysis using 84544-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84544-1-RR (FFAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 84544-1-RR

    IF Staining of mouse brain using 84544-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FFAR2 antibody (84544-1-RR, Clone: 241696B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inTHP-1 cells, HCT 116 cells, HT-29 cells
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84544-1-RR targets FFAR2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name free fatty acid receptor 2
Synonyms GPCR43, G protein coupled receptor 43, free fatty acid receptor 2, FFA2R, FFA2
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC096198
Gene Symbol FFAR2
Gene ID (NCBI) 2867
RRIDAB_3672049
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15552
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

实验方案

Product Specific Protocols
WB protocol for FFAR2 antibody 84544-1-RRDownload protocol
IF protocol for FFAR2 antibody 84544-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols