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验证数据展示

  • IF Staining of PC-3 using 84547-2-RR

    IF Staining of PC-3 using 84547-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ZMPSTE24 antibody (84547-2-RR, Clone: 241288C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84547-2-RR

    Affinity and Kinetic Characterization of 84547-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84547-2-RR against Human ZMPSTE24 were performed. The affinity constant is below 1 pM.

ZMPSTE24 Recombinant antibody

ZMPSTE24 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84547-2-RR

产品说明书

CloneNo. 241288C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84547-2-RR targets ZMPSTE24 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZMPSTE24 fusion protein Ag33613 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc metallopeptidase (STE24 homolog, S. cerevisiae)
别名 FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog
计算分子量 475 aa, 55 kDa
GenBank蛋白编号BC037283
基因名称 ZMPSTE24
Gene ID (NCBI) 10269
RRIDAB_3672054
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75844
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

实验方案

Product Specific Protocols
IF protocol for ZMPSTE24 antibody 84547-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols