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PLTP Recombinant antibody

PLTP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241583F3

Cat no : 84592-1-RR

Print datasheet

Synonyms

Phospholipid transfer protein, Lipid transfer protein II, 241583F3

验证数据展示

  • WB analysis using 84592-1-RR

    WB analysis using 84592-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84592-1-RR

    IHC staining of mouse brain using 84592-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84592-1-RR

    IHC staining of mouse brain using 84592-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84592-1-RR

    Affinity and Kinetic Characterization of 84592-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84592-1-RR is PLTP Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PLTP fusion protein Ag8171 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phospholipid transfer protein
Synonyms Phospholipid transfer protein, Lipid transfer protein II, 241583F3
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC005045
Gene Symbol PLTP
Gene ID (NCBI) 5360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55058
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).

实验方案

Product Specific Protocols
WB protocol for PLTP antibody 84592-1-RRDownload protocol
IHC protocol for PLTP antibody 84592-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols