• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TIM-1 Recombinant antibody

TIM-1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242001D1

Cat no : 84600-5-RR

Print datasheet

Synonyms

HAVCR1, KIM-1, KIM1, HAVcr-1, CD365

验证数据展示

  • WB analysis of HuH-7 using 84600-5-RR

    WB analysis of HuH-7 using 84600-5-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 84600-5-RR (TIM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84600-5-RR

    Affinity and Kinetic Characterization of 84600-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84600-5-RR against Human TIM-1 were performed. The affinity constant is 0.314 nM.

经过测试的应用

Positive WB detected inHuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84600-5-RR targets TIM-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatitis A virus cellular receptor 1
Synonyms HAVCR1, KIM-1, KIM1, HAVcr-1, CD365
Calculated Molecular Weight39kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberNM_001173393.3
Gene Symbol HAVCR1
Gene ID (NCBI) 26762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96D42
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM-1, also known as kidney injury molecule (KIM-1), was first identified as a marker of acute kidney injury. TIM-1 mRNA and protein dramatically increase after ischemic kidney injury while there are low levels in normal kidneys. In addition, TIM-1 is expressed on CD4+ T cells after activation and its expression was sustained preferentially in Th2 but not Th1 cells. However, TIM-1 is expressed on both Th1 and Th2 cells and TIM-1 blockade prolongs survival of fully MHC-mismatched cardiac transplants. TIM-4, expressed by macrophages, is the ligand for TIM-1, and TIM-1 - TIM-4 interactions regulate Th cell responses and modulate Th1/Th2 cytokine balance. Moreover, TIM-1 can regulate macrophage activation and alter the co-stimulatory properties of macrophages. (PMID: 20091883)

实验方案

Product Specific Protocols
WB protocol for TIM-1 antibody 84600-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols