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验证数据展示

  • WB analysis using 84608-5-RR

    WB analysis using 84608-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84608-5-RR (EMR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84608-5-RR

    Affinity and Kinetic Characterization of 84608-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84608-5-RR against Human EMR2 were performed. The affinity constant is 1.45 nM.

EMR2 Recombinant antibody

EMR2 Recombinant Antibody for WB, ELISA
Cat No. 84608-5-RR

产品说明书

CloneNo. 242042E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2, ADGRE2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, THP-1 cells, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84608-5-RR targets EMR2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 egf-like module containing, mucin-like, hormone receptor-like 2
别名 EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2, ADGRE2
计算分子量90 kDa
观测分子量75~90 kDa
GenBank蛋白编号NM_013447.3
基因名称 EMR2
Gene ID (NCBI) 30817
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHX3-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).

实验方案

Product Specific Protocols
WB protocol for EMR2 antibody 84608-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols