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TFEB Recombinant antibody

TFEB Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242093D11

Cat no : 84675-5-RR

Print datasheet

Synonyms

TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB

验证数据展示

  • WB analysis using 84675-5-RR

    WB analysis using 84675-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84675-5-RR

    Affinity and Kinetic Characterization of 84675-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

经过测试的应用

Positive WB detected inRaji cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84675-5-RR targets TFEB in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TFEB fusion protein Ag4194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transcription factor EB
Synonyms TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC032448
Gene Symbol TFEB
Gene ID (NCBI) 7942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

实验方案

Product Specific Protocols
WB protocol for TFEB antibody 84675-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols