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验证数据展示

  • Sandwich ELISA standard curve of MP01497-1

    Sandwich ELISA standard curve of MP01497-1

    Sandwich ELISA standard curve of MP01497-1, Human AEG-1/MTDH Recombinant Matched Antibody Pair - PBS only. 84686-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4840. 84686-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01497-1

    Sample test of MP01497-1

    The mean AEG-1/MTDH concentration was determined to be 77.3 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 84.5 ng/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 80.3 ng/mL in HepG2 cell extract based on a 3.3 mg/mL extract load and 3.8 ng/mL in mouse liver cell extract based on a 3.7 mg/mL extract load.

  • Cytometric bead array standard curve of MP01497-1

    Cytometric bead array standard curve of MP01497-1

    Cytometric bead array standard curve of MP01497-1, AEG-1/MTDH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84686-1-PBS. Detection antibody: 84686-2-PBS. Standard: Ag4840. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01497-2

    Cytometric bead array standard curve of MP01497-2

    Cytometric bead array standard curve of MP01497-2, AEG-1/MTDH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84686-3-PBS. Detection antibody: 84686-2-PBS. Standard: Ag4840. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84686-2-PBS

    Affinity and Kinetic Characterization of 84686-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84686-2-PBS against Human AEG-1 was performed. The affinity constant is 3.43 nM.

AEG-1 Recombinant antibody, PBS Only (Detector)

AEG-1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84686-2-PBS

产品说明书

CloneNo. 242110E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

MTDH, LYRIC, Astrocyte elevated gene-1 protein, AEG1, AEG 1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.

产品信息

84686-2-PBS targets AEG-1 as part of a matched antibody pair:

MP01497-1: 84686-1-PBS capture and 84686-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01497-2: 84686-3-PBS capture and 84686-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 AEG-1 fusion protein Ag4840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 metadherin
别名 MTDH, LYRIC, Astrocyte elevated gene-1 protein, AEG1, AEG 1
计算分子量 582 aa, 64 kDa
GenBank蛋白编号BC045642
基因名称 AEG-1/MTDH
Gene ID (NCBI) 92140
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86UE4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C