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验证数据展示

  • IF Staining of HeLa using 84715-4-RR

    IF Staining of HeLa using 84715-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AGGF1 antibody (84715-4-RR, Clone: 242264F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84715-4-RR

    Affinity and Kinetic Characterization of 84715-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84715-4-RR against Human AGGF1 were performed. The affinity constant is 7.15 nM.

AGGF1 Recombinant antibody

AGGF1 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84715-4-RR

产品说明书

CloneNo. 242264F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7, Angiogenic factor VG5Q

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84715-4-RR targets AGGF1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 AGGF1 fusion protein Ag2497 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiogenic factor with G patch and FHA domains 1
别名 HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7, Angiogenic factor VG5Q
计算分子量 714 aa, 81 kDa
GenBank蛋白编号BC029382
基因名称 AGGF1
Gene ID (NCBI) 55109
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8N302
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.

实验方案

Product Specific Protocols
IF protocol for AGGF1 antibody 84715-4-RRDownload protocol
Standard Protocols
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