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验证数据展示

  • WB analysis using 84727-5-RR

    WB analysis using 84727-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig thymus using 84727-5-RR

    WB analysis of pig thymus using 84727-5-RR

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84727-5-RR

    Affinity and Kinetic Characterization of 84727-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

TMIGD2/CD28H Recombinant antibody

TMIGD2/CD28H Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84727-5-RR

产品说明书

CloneNo. 241807C12

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, ELISA

别名

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, pig thymus tissue, MOLT-4 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84727-5-RR targets TMIGD2/CD28H in WB, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane and immunoglobulin domain containing 2
别名 TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H
计算分子量 282 aa, 31 kDa
观测分子量48 kDa
GenBank蛋白编号BC015655
基因名称 TMIGD2
Gene ID (NCBI) 126259
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96BF3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).

实验方案

Product Specific Protocols
WB protocol for TMIGD2/CD28H antibody 84727-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols