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验证数据展示

  • WB analysis using 84762-3-RR

    WB analysis using 84762-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84762-3-RR (CACNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84762-3-RR

    WB analysis using 84762-3-RR

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 84762-3-RR (CACNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84762-3-RR

    IHC staining of mouse brain using 84762-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84762-3-RR (CACNB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84762-3-RR

    Affinity and Kinetic Characterization of 84762-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84762-3-RR against Human CACNB3 were performed. The affinity constant is 0.241 nM.

CACNB3 Recombinant monoclonal antibody

CACNB3 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84762-3-RR

产品说明书

CloneNo. 242088G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

242088G1, CAB3, CACNB 3, CACNLB3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells, SKOV-3 cells, SW480 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84762-3-RR targets CACNB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35651

Product name: Recombinant human CACNB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-484 aa of NM_000725.3

Sequence: EYLEVYWRATHHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQRSSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWPKDSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium channel, voltage-dependent, beta 3 subunit
别名 242088G1, CAB3, CACNB 3, CACNLB3
计算分子量55kDa，484aa
观测分子量58 kDa
GenBank蛋白编号NM_000725.3
基因名称 CACNB3
Gene ID (NCBI) 784
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACNB3, also known as CACNLB3, is a voltage-gated Ca2+ channel subunit. It has 5 isoforms and may play a role in the regulation of transcription factors and calcium transport.

实验方案

Product Specific Protocols
IHC protocol for CACNB3 antibody 84762-3-RRDownload protocol
WB protocol for CACNB3 antibody 84762-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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