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验证数据展示

  • WB analysis using 84803-4-RR

    WB analysis using 84803-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 84803-4-RR

    IP experiment of HEK-293 using 84803-4-RR

    IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug.

  • Affinity and Kinetic Characterization of 84803-4-RR

    Affinity and Kinetic Characterization of 84803-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

ZDHHC5 Recombinant antibody

ZDHHC5 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 84803-4-RR

产品说明书

CloneNo. 242192A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Zinc finger DHHC domain-containing protein 5, Palmitoyltransferase ZDHHC5, KIAA1748, EC:2.3.1.225, DHHC-5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, mouse brain tissue
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84803-4-RR targets ZDHHC5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 ZDHHC5 fusion protein Ag15022 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger, DHHC-type containing 5
别名 Zinc finger DHHC domain-containing protein 5, Palmitoyltransferase ZDHHC5, KIAA1748, EC:2.3.1.225, DHHC-5
计算分子量 715 aa, 78 kDa
观测分子量 75-77 kDa
GenBank蛋白编号BC026967
基因名称 ZDHHC5
Gene ID (NCBI) 25921
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9C0B5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

实验方案

Product Specific Protocols
WB protocol for ZDHHC5 antibody 84803-4-RRDownload protocol
IP protocol for ZDHHC5 antibody 84803-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols