P62,SQSTM1 Recombinant antibody

P62,SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 84826-1-RR
CloneNo. 241992C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

P62/SQSTM1, p62, Sqstm1, sequestosome 1, Osi

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 μg/ml


经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, rat brain tissue, HepG2 cells, Jurkat cells, Raji cells, HEK-293T cells, MCF-7 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84826-1-RR targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen P62,SQSTM1 fusion protein Ag35064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sequestosome 1
Synonyms P62/SQSTM1, p62, Sqstm1, sequestosome 1, Osi
Calculated Molecular Weight48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberNM_011018
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 18412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ64337
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 84826-1-RRDownload protocol
IHC protocol for P62,SQSTM1 antibody 84826-1-RRDownload protocol
IF protocol for P62,SQSTM1 antibody 84826-1-RRDownload protocol
IP protocol for P62,SQSTM1 antibody 84826-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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