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PISD Recombinant antibody, PBS Only (Detector)

PISD Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242280C9

Cat no : 84866-2-PBS

Print datasheet

Synonyms

PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65

验证数据展示

  • WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2, Human PISD Recombinant Matched Antibody Pair - PBS only. 84866-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9499. 84866-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01640-2

    Sample test of MP01640-2

    The mean PISD concentration was determined to be 19.97 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

  • Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1, PISD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84866-1-PBS. Detection antibody: 84866-2-PBS. Standard: Ag9499. Range: 0.781-50 ng/mL.

产品信息

84866-2-PBS targets PISD as part of a matched antibody pair:

MP01640-1: 84866-1-PBS capture and 84866-2-PBS detection (validated in Cytometric bead array)

MP01640-2: 84866-3-PBS capture and 84866-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, rat
Immunogen PISD fusion protein Ag9499 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylserine decarboxylase
Synonyms PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65
Calculated Molecular Weight375aa,43 kDa; 408aa,46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001482
Gene Symbol PISD
Gene ID (NCBI) 23761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UG56
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.