• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2, Human PISD Recombinant Matched Antibody Pair - PBS only. 84866-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9499. 84866-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01640-2

    Sample test of MP01640-2

    The mean PISD concentration was determined to be 19.97 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

  • Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1, PISD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84866-1-PBS. Detection antibody: 84866-2-PBS. Standard: Ag9499. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 84866-2-RR

    Affinity and Kinetic Characterization of 84866-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84866-2-RR against Human PISD were performed. The affinity constant is 0.441 nM.

PISD Recombinant antibody, PBS Only (Detector)

PISD Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84866-2-PBS

产品说明书

CloneNo. 242280C9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84866-2-PBS targets PISD as part of a matched antibody pair:

MP01640-1: 84866-1-PBS capture and 84866-2-PBS detection (validated in Cytometric bead array)

MP01640-2: 84866-3-PBS capture and 84866-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, rat
免疫原 PISD fusion protein Ag9499 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylserine decarboxylase
别名 PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65
计算分子量375aa,43 kDa; 408aa,46 kDa
观测分子量46 kDa
GenBank蛋白编号BC001482
基因名称 PISD
Gene ID (NCBI) 23761
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UG56
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.