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CHKA Recombinant antibody

CHKA Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242095B4

Cat no : 84881-2-RR

Print datasheet

Synonyms

EC:2.7.1.32, CKI, choline kinase alpha, CHK, CHETK-alpha

验证数据展示

  • WB analysis using 84881-2-RR

    WB analysis using 84881-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84881-2-RR (CHKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84881-2-RR

    Affinity and Kinetic Characterization of 84881-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84881-2-RR against Human CHKA were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84881-2-RR targets CHKA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CHKA fusion protein Ag4761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name choline kinase alpha
Synonyms EC:2.7.1.32, CKI, choline kinase alpha, CHK, CHETK-alpha
Calculated Molecular Weight 439 aa, 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC036471
Gene Symbol CHKA
Gene ID (NCBI) 1119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CHKA antibody 84881-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols