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验证数据展示

  • IHC staining of mouse spleen using 84882-5-RR

    IHC staining of mouse spleen using 84882-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84882-5-RR

    IHC staining of mouse spleen using 84882-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84882-5-RR

    IF Staining of HeLa using 84882-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84882-5-RR

    Affinity and Kinetic Characterization of 84882-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84882-5-RR against Human BCL3 were performed. The affinity constant is 0.581 nM.

BCL3 Recombinant antibody

BCL3 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84882-5-RR

产品说明书

CloneNo. 242233B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Proto-oncogene BCL3, D19S37, BCL4, BCL-3, B-cell lymphoma 3 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84882-5-RR targets BCL3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BCL3 fusion protein Ag13375 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 3
别名 Proto-oncogene BCL3, D19S37, BCL4, BCL-3, B-cell lymphoma 3 protein
计算分子量 446 aa, 47 kDa
观测分子量 48-60 kDa
GenBank蛋白编号BC064993
基因名称 BCL3
Gene ID (NCBI) 602
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).

实验方案

Product Specific Protocols
IHC protocol for BCL3 antibody 84882-5-RRDownload protocol
IF protocol for BCL3 antibody 84882-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols