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验证数据展示

  • WB analysis using 84884-5-RR

    WB analysis using 84884-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84884-5-RR (MTHFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 84884-5-RR

    IF Staining of HEK-293 using 84884-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (84884-5-RR, Clone: 242388F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84884-5-RR

    Affinity and Kinetic Characterization of 84884-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84884-5-RR against Human MTHFD1 were performed. The affinity constant is below 1 pM.

MTHFD1 Recombinant antibody

MTHFD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84884-5-RR

产品说明书

CloneNo. 242388F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242388F1, C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HuH-7 cells, HEK-293 cells, A549 cells, MCF-7 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84884-5-RR targets MTHFD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MTHFD1 fusion protein Ag1225 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
别名 242388F1, C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein
计算分子量 101 kDa
观测分子量 101-110 kDa
GenBank蛋白编号BC009806
基因名称 MTHFD1
Gene ID (NCBI) 4522
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP11586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

实验方案

Product Specific Protocols
WB protocol for MTHFD1 antibody 84884-5-RRDownload protocol
IF protocol for MTHFD1 antibody 84884-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols