验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84893-5-RR targets ARK5 in IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ARK5 fusion protein Ag18832 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NUAK family, SNF1-like kinase, 1 |
| 别名 | NUAK1, NUAK family SNF1-like kinase 1, NUAK 1, EC:2.7.11.1, AMPK-related protein kinase 5 |
| 计算分子量 | 661 aa, 74 kDa |
| GenBank蛋白编号 | BC152462 |
| 基因名称 | ARK5 |
| Gene ID (NCBI) | 9891 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O60285 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARK5 antibody 84893-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |