验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, COLO 320 cells, NCI-H1299 cells, mouse colon tissue, rat colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84900-1-RR targets RPL15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RPL15 fusion protein Ag9948 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L15 |
| 别名 | RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45, 60S ribosomal protein L15 |
| 计算分子量 | 204 aa, 24 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC070328 |
| 基因名称 | RPL15 |
| Gene ID (NCBI) | 6138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P61313 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL15 (ribosomal protein L15) is a component of the ribosomal large subunit 60S, participating in the assembly process of ribosomal subunits and involved in the processing of rRNA. Moreover, RPL15 deletion will lead to abnormalities in the biogenesis of ribosomal subunits. The abnormal expression or mutation of RPL15 protein is also closely related to the occurrence and development of many diseases. RPL15 was highly expressed in gastric cancer cell lines, and interference with the expression of RPL15 could inhibit gastric cancer cell growth. In addition, RPL15 in skin squamous cell carcinoma tissues and pancreatic cancer cell lines decreased sharply, which had a great relationship with the survival rate. PMID:35410346
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL15 antibody 84900-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |