Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibody
Di/Tri-Methyl-Histone H3 (Lys4) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, Dot Blot, ELISA
Conjugate
Unconjugated
CloneNo.
241942D11
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse testis tissue, mouse liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84908-2-RR targets Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, Dot Blot, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | histone cluster 1, H3a |
| Synonyms | HIST1H3A, 241942D11, Histone-H3, HistoneH3, Histone H3 |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RR | Download protocol |
| IHC protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RR | Download protocol |
| IF protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |