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验证数据展示

  • WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

    WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84943-2-RR (APOH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1, Human APOH Recombinant Matched Antibody Pair - PBS only. 84943-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3084. 84943-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Plasma of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 51.16 µg/mL with a range of 38.03 - 72.18 µg/mL.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    The mean APOH concentration was determined to be 21.74 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Serum of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 69.62 µg/mL with a range of 55.50 - 88.74 µg/mL.

  • Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1, APOH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84943-1-PBS. Detection antibody: 84943-2-PBS. Standard: Eg3084. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84943-2-RR

    Affinity and Kinetic Characterization of 84943-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84943-2-RR against Human APOH were performed. The affinity constant is below 1 pM.

APOH Recombinant antibody, PBS Only (Detector)

APOH Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84943-2-PBS

产品说明书

CloneNo. 242454E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.

产品信息

84943-2-PBS targets APOH as part of a matched antibody pair:

MP01667-1: 84943-1-PBS capture and 84943-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein H (beta-2-glycoprotein I)
别名 Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
计算分子量38 kDa
观测分子量 43-55 kDa
GenBank蛋白编号NM_000042.2
基因名称 APOH
Gene ID (NCBI) 350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02749
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.