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验证数据展示

  • WB analysis of HUVEC using 84945-4-RR

    WB analysis of HUVEC using 84945-4-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 84945-4-RR (CD93 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84945-4-RR

    IHC staining of human tonsillitis using 84945-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84945-4-RR (CD93 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84945-4-RR

    IHC staining of human tonsillitis using 84945-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84945-4-RR (CD93 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD93 Recombinant antibody

CD93 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84945-4-RR

产品说明书

CloneNo. 242464D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C1QR1, Matrix-remodeling-associated protein 4, C1qRp, C1qR(p), C1qR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84945-4-RR targets CD93 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD93 molecule
别名 C1QR1, Matrix-remodeling-associated protein 4, C1qRp, C1qR(p), C1qR
计算分子量69 kDa
观测分子量120 kDa
GenBank蛋白编号NM_012072.3
基因名称 CD93
Gene ID (NCBI) 22918
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NPY3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD93, also known as C1qR or C1qR(p), is a single-pass type I transmembrane glycoprotein and a member of the group 14 of the C-type lectin-like domain (CTLD) superfamily, which also includes endosialin/CD248, thrombomodulin, and CLEC14A (PMID: 31287944). CD93 consists of a C-type lectin-like domain, five EGF-like repeats, a mucin-like domain, a transmembrane domain and a cytoplasmic domain (PMID: 28912033). CD93 is mainly expressed on endothelial cells and has been reported to be expressed by neurons and various cells of the hematopoietic system, including monocytes, neutrophils, B cells, natural killer cells, platelets and hematopoietic stem cells (PMID: 37443812). CD93 plays a role in various physiological processes including angiogenesis, inflammation, and cell adhesion (PMID: 31287944).

实验方案

Product Specific Protocols
WB protocol for CD93 antibody 84945-4-RRDownload protocol
IHC protocol for CD93 antibody 84945-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols