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验证数据展示

  • WB analysis using 84951-4-RR

    WB analysis using 84951-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84951-4-RR

    WB analysis using 84951-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84951-4-RR (GABPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 84951-4-RR

    IHC staining of human cervical cancer using 84951-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GABPA antibody included
  • IHC staining of human cervical cancer using 84951-4-RR

    IHC staining of human cervical cancer using 84951-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84951-4-RR (GABPA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GABPA antibody included
  • IF Staining of NIH/3T3 using 84951-4-RR

    IF Staining of NIH/3T3 using 84951-4-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GABPA antibody (84951-4-RR, Clone: 242578F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84951-4-RR

    Affinity and Kinetic Characterization of 84951-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84951-4-RR against Human GABPA were performed. The affinity constant is 2.51 nM.

GABPA Recombinant antibody

GABPA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84951-4-RR

产品说明书

CloneNo. 242578F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, K-562 cells, HuH-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy GABPA Ready-To-Use IHC Kit. GABPA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84951-4-RR targets GABPA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GABPA fusion protein Ag16191 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GA binding protein transcription factor, alpha subunit 60kDa
别名 242578F10, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain
计算分子量 454 aa, 51 kDa
观测分子量 56-60 kDa
GenBank蛋白编号BC035031
基因名称 GABPA
Gene ID (NCBI) 2551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ06546
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

实验方案

Product Specific Protocols
WB protocol for GABPA antibody 84951-4-RRDownload protocol
IHC protocol for GABPA antibody 84951-4-RRDownload protocol
IF protocol for GABPA antibody 84951-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols