• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Sandwich ELISA standard curve of MP01703-3

    Sandwich ELISA standard curve of MP01703-3

    Sandwich ELISA standard curve of MP01703-3, Human USP33 Recombinant Matched Antibody Pair - PBS only. 84958-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14268. 84958-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01703-3

    Sample test of MP01703-3

    The mean USP33 concentration was determined to be 8.27 ng/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load, 4.70 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 3.49 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load.

  • IF Staining of HeLa using 84958-1-RR (same clone as 84958-1-PBS)

    IF Staining of HeLa using 84958-1-RR (same clone as 84958-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP33 antibody (84958-1-RR, Clone: 242535A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84958-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01703-2

    Cytometric bead array standard curve of MP01703-2

    Cytometric bead array standard curve of MP01703-2, USP33 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84958-2-PBS. Detection antibody: 84958-1-PBS. Standard: Ag14268. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01703-1

    Cytometric bead array standard curve of MP01703-1

    Cytometric bead array standard curve of MP01703-1, USP33 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84958-1-PBS. Detection antibody: 84958-3-PBS. Standard: Ag14268. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84958-1-RR

    Affinity and Kinetic Characterization of 84958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84958-1-RR against Human USP33 were performed. The affinity constant is below 1 pM.

USP33 Recombinant antibody, PBS Only (Capture/Detector)

USP33 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84958-1-PBS

产品说明书

CloneNo. 242535A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1, EC:3.4.19.12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84958-1-PBS targets USP33 as part of a matched antibody pair:

MP01703-1: 84958-1-PBS capture and 84958-3-PBS detection (validated in Cytometric bead array)

MP01703-2: 84958-2-PBS capture and 84958-1-PBS detection (validated in Cytometric bead array)

MP01703-3: 84958-1-PBS capture and 84958-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 USP33 fusion protein Ag14268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 33
别名 Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1, EC:3.4.19.12
计算分子量 942 aa, 107 kDa
GenBank蛋白编号BC016663
基因名称 USP33
Gene ID (NCBI) 23032
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TEY7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.