验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, L02 cells, mouse liver tissue, rat liver tissue, human placenta tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84962-4-RR targets GOSR2/Membrin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | GOSR2/Membrin fusion protein Ag2736 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | golgi SNAP receptor complex member 2 |
| 别名 | Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein |
| 计算分子量 | 212 aa, 25 kDa |
| 观测分子量 | 23-25 kDa |
| GenBank蛋白编号 | BC009710 |
| 基因名称 | Membrin |
| Gene ID (NCBI) | 9570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O14653 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the soluble NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in the Golgi apparatus. It is involved in the transport of proteins from the cis/medial-Golgi to the trans-Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GOSR2/Membrin antibody 84962-4-RR | Download protocol |
| IF protocol for GOSR2/Membrin antibody 84962-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |