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验证数据展示

  • WB analysis using 84971-3-RR

    WB analysis using 84971-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 84971-3-RR

    IHC staining of human stomach cancer using 84971-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 84971-3-RR

    IHC staining of human stomach cancer using 84971-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 84971-3-RR

    IF Staining of NIH/3T3 using 84971-3-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84971-3-RR

    Affinity and Kinetic Characterization of 84971-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84971-3-RR against Human GS28 were performed. The affinity constant is 2.23 nM.

GS28 Recombinant antibody

GS28 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84971-3-RR

产品说明书

CloneNo. 242552G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GOSR1, GOS-28, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SK-BR-3 cells, NIH/3T3 cells, C6 cells, PC-12 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84971-3-RR targets GS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GS28 fusion protein Ag9021 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi SNAP receptor complex member 1
别名 GOSR1, GOS-28, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28
计算分子量28.6 kDa
观测分子量 28 kDa
GenBank蛋白编号BC012620
基因名称 GS28
Gene ID (NCBI) 9527
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

实验方案

Product Specific Protocols
WB protocol for GS28 antibody 84971-3-RRDownload protocol
IHC protocol for GS28 antibody 84971-3-RRDownload protocol
IF protocol for GS28 antibody 84971-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols