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Phospho-EIF4E (Ser209) Recombinant antibody

Phospho-EIF4E (Ser209) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242049G10

Cat no : 84981-1-RR

Print datasheet

Synonyms

EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209

验证数据展示

  • WB analysis of HEK-293T using 84981-1-RR

    WB analysis of HEK-293T using 84981-1-RR

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84981-1-RR targets Phospho-EIF4E (Ser209) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 4E
Synonyms EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209
Calculated Molecular Weight 29 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)

实验方案

Product Specific Protocols
WB protocol for Phospho-EIF4E (Ser209) antibody 84981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols