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验证数据展示

  • IF Staining of PC-12 using 84984-1-RR

    IF Staining of PC-12 using 84984-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RNF135 antibody (84984-1-RR, Clone: 241606D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84984-1-RR

    Affinity and Kinetic Characterization of 84984-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84984-1-RR against Human RNF135 were performed. The affinity constant is 49.1 nM.

RNF135 Recombinant antibody

RNF135 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 84984-1-RR

产品说明书

CloneNo. 241606D8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, ELISA

别名

RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF135

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84984-1-RR targets RNF135 in IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 RNF135 fusion protein Ag18821 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 135
别名 RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF135
计算分子量 432 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC005084
基因名称 RNF135
Gene ID (NCBI) 84282
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IUD6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication.

实验方案

Product Specific Protocols
IF protocol for RNF135 antibody 84984-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols