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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84996-1-RR (NADK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84996-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01732-1

    Cytometric bead array standard curve of MP01732-1

    Cytometric bead array standard curve of MP01732-1, NADK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84996-1-PBS. Detection antibody: 84996-2-PBS. Standard: Ag7906. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84996-1-RR

    Affinity and Kinetic Characterization of 84996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84996-1-RR against Human NADK were performed. The affinity constant is 2.25 nM.

NADK Recombinant antibody, PBS Only (Capture)

NADK Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84996-1-PBS

产品说明书

CloneNo. 242502B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23, 242502B7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84996-1-PBS targets NADK as part of a matched antibody pair:

MP01732-1: 84996-1-PBS capture and 84996-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NADK fusion protein Ag7906 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NAD kinase
别名 Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23, 242502B7
计算分子量 49 kDa
观测分子量 49-52 kDa
GenBank蛋白编号BC001709
基因名称 NADK
Gene ID (NCBI) 65220
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95544
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).