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验证数据展示

  • WB analysis using 85041-3-RR

    WB analysis using 85041-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85041-3-RR (HIRIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 85041-3-RR

    WB analysis of HEK-293 using 85041-3-RR

    WB result of HIRIP3 antibody (85041-3-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HEK-293 cells.

  • IF Staining of HeLa using 85041-3-RR

    IF Staining of HeLa using 85041-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRIP3 antibody (85041-3-RR, Clone: 242708G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  • KD/KO验证

HIRIP3 Recombinant monoclonal antibody

HIRIP3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85041-3-RR

产品说明书

CloneNo. 242708G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

242708G7, HIRA-interacting protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HIRIP3 Ready-To-Use IHC Kit. HIRIP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85041-3-RR targets HIRIP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6978

Product name: Recombinant human HIRIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 207-556 aa of BC000588

Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HIRA interacting protein 3
别名 242708G7, HIRA-interacting protein 3
计算分子量 62 kDa
观测分子量 90 kDa
GenBank蛋白编号BC000588
基因名称 HIRIP3
Gene ID (NCBI) 8479
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

实验方案

Product Specific Protocols
IF protocol for HIRIP3 antibody 85041-3-RRDownload protocol
WB protocol for HIRIP3 antibody 85041-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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