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验证数据展示

  • WB analysis using 85060-2-RR

    WB analysis using 85060-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85060-2-RR (SLC6A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 85060-2-RR

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 85060-2-RR

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 85060-2-RR

    IHC staining of rat brain using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85060-2-RR

    Affinity and Kinetic Characterization of 85060-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85060-2-RR against Human GAT1 were performed. The affinity constant is below 1 pM.

GAT1 Recombinant antibody

GAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85060-2-RR

产品说明书

CloneNo. 242637D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SLC6A1, GAT 1, GABT1, GABATR, GABATHG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85060-2-RR targets GAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GAT1 fusion protein Ag29712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, GABA), member 1
别名 SLC6A1, GAT 1, GABT1, GABATR, GABATHG
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC033904
基因名称 GABA Transporter 1/GAT1
Gene ID (NCBI) 6529
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30531
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GAT1 antibody 85060-2-RRDownload protocol
IHC protocol for GAT1 antibody 85060-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols