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验证数据展示

  • WB analysis using 85110-4-RR

    WB analysis using 85110-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85110-4-RR (SEC13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 85110-4-RR

    IF Staining of HepG2 using 85110-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC13 antibody (85110-4-RR, Clone: 242775C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85110-4-RR

    Affinity and Kinetic Characterization of 85110-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85110-4-RR against Human SEC13 were performed. The affinity constant is 0.114 nM.

SEC13 Recombinant antibody

SEC13 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85110-4-RR

产品说明书

CloneNo. 242775C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HepG2 cells, A431 cells, RAW 264.7 cells, mouse pancreas tissue, rat pancreas tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85110-4-RR targets SEC13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEC13 fusion protein Ag7610 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC13 homolog (S. cerevisiae)
别名 SEC13L1, SEC13A, Protein SEC13 homolog, GATOR2 complex protein SEC13, D3S1231E
计算分子量 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC002634
基因名称 SEC13
Gene ID (NCBI) 6396
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

实验方案

Product Specific Protocols
WB protocol for SEC13 antibody 85110-4-RRDownload protocol
IF protocol for SEC13 antibody 85110-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols