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验证数据展示

  • WB analysis using 85133-3-RR

    WB analysis using 85133-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 85133-3-RR

    WB analysis of DU 145 using 85133-3-RR

    DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 85133-3-RR

    IHC staining of mouse cerebellum using 85133-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 85133-3-RR

    IF Staining of mouse brain using 85133-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Kv4.2 Recombinant antibody

Kv4.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85133-3-RR

Print datasheet

CloneNo. 242754F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KCND2, 242754F4, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, A549 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85133-3-RR targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Kv4.2 fusion protein Ag15879 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name potassium voltage-gated channel, Shal-related subfamily, member 2
Synonyms KCND2, 242754F4, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC110449
Gene Symbol KCND2
Gene ID (NCBI) 3751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

实验方案

Product Specific Protocols
WB protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IHC protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IF protocol for Kv4.2 antibody 85133-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols