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验证数据展示

  • WB analysis using 85142-2-RR (same clone as 85142-2-PBS)

    WB analysis using 85142-2-RR (same clone as 85142-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85142-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01841-2

    Sandwich ELISA standard curve of MP01841-2

    Sandwich ELISA standard curve of MP01841-2, Humanc NCKAP1 Recombinant Matched Antibody Pair - PBS only. 85142-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2788. 85142-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • Sample test of MP01841-2

    Sample test of MP01841-2

    The mean NCKAP1 concentration was determined to be 38.80 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 46.44 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01841-2

    Cytometric bead array standard curve of MP01841-2

    Cytometric bead array standard curve of MP01841-2, NCKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85142-3-PBS. Detection antibody: 85142-2-PBS. Standard: Ag2788. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP01841-1

    Cytometric bead array standard curve of MP01841-1

    Cytometric bead array standard curve of MP01841-1, NCKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85142-1-PBS. Detection antibody: 85142-2-PBS. Standard: Ag2788. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 85142-2-RR

    Affinity and Kinetic Characterization of 85142-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

NAP1;NCKAP1 Recombinant antibody, PBS Only (Detector)

NAP1;NCKAP1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85142-2-PBS

产品说明书

CloneNo. 242784C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85142-2-PBS targets NAP1;NCKAP1 as part of a matched antibody pair:

MP01841-1: 85142-1-PBS capture and 85142-2-PBS detection (validated in Cytometric bead array)

MP01841-2: 85142-3-PBS capture and 85142-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 NAP1;NCKAP1 fusion protein Ag2788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NCK-associated protein 1
别名 NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587
计算分子量 129 kDa
观测分子量 115-125 kDa
GenBank蛋白编号BC015025
基因名称 NCKAP1
Gene ID (NCBI) 10787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2A7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.